SIM Scenario Exchange

DAT Positive Simulation

Contributed on July 4, 2022, last updated February 10, 2023.



Amanda VanSpronsen, University of Alberta

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The player is a student technologist on practicum at a clinical site.
Due to the blood bank being abnormally busy, the preceptor gives the student access to the LIS, reagents, and SOPs.
A series of levels will parallel mastery learning of basic to more difficult concepts and include interpretation, technical errors, understanding of the method, interferences, troubleshooting and more.

Learning Objectives:

State the purpose and principle of the direct antiglobulin test (DAT) (3.16)

State the clinical situations when a DAT would be performed and explain the potential results while taking into consideration patient history and other clinical, and laboratory findings (4.01)

Describe the quality control, routine follow-up, and limitations of DAT testing. (5.03, 3.16.02, 3.16.03, 3.16.04)

Identifies interferences in DAT testing that may lead to erroneous results (5.05)

Recognizes errors in methods, equipment and reagents, and suggests appropriate trouble-shooting strategies (5.07, 6.06)

Practices effective communication and conflict management strategies when working with other healthcare professionals (7.01, 7.03)

Simulation Modality:

Virtual gaming simulation / virtual patient


This simulation’s scenario and script were formally peer-reviewed by two external institutions, then the final simulation was pilot tested by several students on a student testing committee then as part of the Virtu-WIL Student Program’s rollout.


060, Affinity Learning, Lab procedures


VanSpronsen A. DAT Positive Simulation. Simulation resource published by Simulation Canada; 2022. Available from

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Target Learners




Medical Laboratory Science


Undergraduate / post-secondary

Fields / Disciplines:

Transfusion Science

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